Application

Use Anti-BAF (BANF1) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect BAF (BANF1) also known as 53 kDa BRG1-associated factor A.

Western Blot Analysis: A 1:1,000 dilution from a previous lot detected BAF in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, NIH/3T3, PC12, PC3, and RAW264.7 cell lysates.

General description

Barrier-to-auto integration factor (BAF or BANF1) plays fundamental roles in nuclear assembly, chromatin organization, gene expression and development. BAF may potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. BAF contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. BAF is exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. BAF is found in both the nucleus and cytoplasm and is significantly enriched at the nuclear inner membrane, diffused throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase.

Immunogen

KLH-conjugated linear peptide corresponding to human BAF (BANF1).

Quality

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected BAF in 10 µg of HeLa cell lysate.

Specificity

This antibody recognizes BAF (BANF1).

Target description

~10 kDa